#Western blot quantification with imagej how to#

So, when I tried to draw a bigger rectangular this problem stopped! Of course the width of your rectangular should be made according to the band size, so change the height and make it a little taller. This method is the Gel Analysis method outlined in the ImageJ documentation: Gel. The signal intensity of the band is directly. By running a mixture of polypeptides of known molecular weights (standards), one can construct a standard curve showing this.

#Western blot quantification with imagej how to#

I had the same problem with Imagej and the solution came by coincidence! I realized that if I draw a rectangular with a width that is longer than twice the size of the height, then this thing happens. How to use imagej for western blot quantification. Quantitating a western blot refers to the measurement of the signal emitted by your protein band(s) of interest. I have 1.41 version and there is no Outline Lanes in Gel Analyzer Options.ĭoes anabody know how to fight this problem? Maybe someone has an earlier version and can send it to me I have to say that sometimes it does work properlý, but mostly it does not and I do not see any order in this. Unfortunately ít stays not on the second band but jumps back to the first. About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy & Safety How YouTube works Test new features NFL Sunday Ticket Press Copyright. Then I move the rectangular to the second band press 2 (or Ctrl 2, works the same). So I was wondering how do you actually set the cut offs in this case. I open the file (.tiff), select rectangular, press 1 or Ctrl 1 to select the first lane.

I have a problem with ImageJ analysing western blots.